![]() Never forget the first day in that same cop car alone and how everything looked foreign, people looked twice as big and you don't know how but some smart ass was able to move all the streets around from where they were last week and now you're lost. Never forget your first arrest, report, scuffle, or ass-chewing. Never forget your first time in the car trying to figure out where to put your stuff while listening to a radio with 5 different channels and 20 different voices all while trying to remember all the faces, cars and storefronts that pass you all while listening to some person you just met telling you how a mistake out here can cost you or someone their life. Never forget your first time in briefing. Never forget your awkward and bulky ride to work. Never forget the night before your first day. Never forget your first time in uniform and the awkward bulkiness.Don’t be afraid to speak up when you know something is being done wrong even if “that’s how we’ve always done it.” Read and be able to explain case law and code. Although most senior officers and supervisors mean well, they aren’t always right.Having good supportive leadership and administration in your agency is more important than high pay, technology and fancy gadgets.Use it to help you remember the victims and some of the funniest things you can imagine, and some that defy your imagination. Use it to memorialize leadership wins and failures. Use that journal for reflection and personal growth. You have a front-row seat to the greatest show on Earth and it will all become a blur after the first few years. What is the public’s perception of how I am doing my job? It will determine their support for you when you need it most. What are my peers’ perceptions of my abilities and limitations? They affect how they do their job. What are my abilities and my limitations? They affect how I do my job. Ask yourself these three questions regularly, and answer them with ruthless honesty: 1.Always give yourself an advantage in every situation, do what has to be done, and take care of yourself, your partner, other officers and the people you serve, but the goal at the end of the day is to make it home! Hold your family tight, and spend as much time with them as you can – working OT, or off duty is great, but you won’t get the time with your family back. This job will eat you up if you let it, it’s not a crime to take a minute for yourself, or a week if you need it, you have to stay mentally healthy, or you will not last! When you make a mistake, and we all do, give yourself some grace, but always learn from that mistake. There is more to life than cop stuff! This is your career, not your life! And most importantly, read Kevin Gilmartin’s book “Emotional Survival.” Don’t eat, sleep and breath police work no matter what your peers think. – Rodney Scottĭon’t be peer pressured into being “on duty” 24/7. Keep the humor because it is going to be a wild ride.As my FTO told me and I didn’t quite take to heart, “It’s a career, not a crusade!” – Blair Powers.Not every lesson has to be learned the hard way.Wake up, sit up and pay attention because it might just save your or a buddy’s life one day. ![]()
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![]() ![]() When the cyst bursts the new parasites will swim through the water to find a fish to feed from, the time when they are swimming is the time the treatment will attack. Most of the tetras will thrive in moderately warm tanks with strong water currents. ![]() After the parasites have stopped feeding from the fish they will drop and begin to duplicate in a cyst on the bottom of the tank. ![]() Finally, scrubbing with a brush can effectively remove dirt and debris from a fish tank. Pour boiling water into the tank and wait 10 minutes before doing a full clean-up. Boiling water can also use to clean a fish tank. White spot treatments require two doses to catch the parasites at their most delicate stage. This will break down the proteins and bacteria that may have caused the buildup of jelly balls. The white spots you see on the fish is the mature stage of the parasites life cycle and will not be directly affected by treatment. As stress factors in the tank weaken the fish, parasites can reach dangerous numbers and will become harmful to their fish hosts.Ĭontrolling stress factors is key to preventing outbreaks and the recovery of your fish. Parasites are naturally present in aquariums and will be happily controlled by the immune system of your fish in small numbers. Often referred to as Itch or Ick, White spot is caused by the Ichyophirius parasite. How to treat high ammonia in an aquarium.How to treat high nitrite in an aquarium.How to treat high nitrate in an aquarium.How to solve green water in an aquarium.How to maintain water levels in an aquarium.How to remove hair algae from an aquarium.How to solve cloudy water in an aquarium.How to resolve plant problems in an aquarium.How to treat Fungus and Bacteria in aquarium fish.How to treat Internal Bacteria in aquarium fish.How to treat Invisible Parasites in aquarium fish.How to treat White Spot in aquarium fish.1 This mucus allows the fish to poop, and it is always present in your fish’s feces. Like most animals, fish have cells in the gut that produce mucus that acts as lubrication, allowing the contents of their stomachs to slide through unencumbered. How to treat a Visible Parasite in aquarium fish Sometimes the white stuff floating in your fish tank is fecal matter.How to treat Body Deformities in aquarium fish.How to treat a Swim Bladder Infection in aquarium fish.Behaving unwell but no physical symptoms.Like all tetras, glowlight tetras can survive in different aquarium environments. The glowlight tetra is native to the Guyana river in South America and is active, colorful, easy to care for, and fun to watch. How to treat unexplained fish death in an aquarium The glowlight tetra is a beautiful freshwater fish.pH Adjuster Down for Alkaline Aquariums.That will help you find ideal tank mate pairings and learn more about each species. If you’re stuck or have any questions, we highly recommend checking out the individual care guides we’ve linked to throughout the list. translucent filaments attached near its pectoral fins, and which streamed. Since all of the fish on our list are absolutely fantastic, it all comes down to your personal preference and your vision for the tank. (who first described the common aquarium fish called the neon tetra). Now that you’re familiar with all of the best community fish for your freshwater aquarium, it’s time to pick which ones you like the most. As long as you avoid any large or aggressive species, the glass catfish will thrive in your freshwater tank. There are plenty of options available to you when planning out what other species you want to keep with the glass catfish. They are quite an active species, but won’t cause any trouble with other tank mates. They are fairly low-maintenance fish to care for and will do quite well in community tanks. Dropsy In Fish: Your Guide To Symptoms & Treatment.Why Is My Goldfish Turning White? All Possible Causes.Why Your Betta Fish Is Laying At The Bottom Of The Tank. ![]() Overall it offers a better reading experience if you are an avid reader. There is also a built-in scanner which you can use to convert any document into PDF and you can also convert PDF to different file formats. You can also access the PDFs from iCloud Drive, Google Drive, Dropbox, OneDrive and Box using the app. It allows you to read, edit, annotate, convert and even sign PDF files. PDFelement is a really good PDF reader app which provides the best cross-platform syncing. Multi-tab view to manage different files Real-time collaboration to annotate PDF files Support change of text size, crop images Fast PDF viewing engine with smooth navigation It also allows multiple users to access the documents and collaborate on the document. ![]() The app also features the really good cross-platform support which is another major plus. Just like other readers, you can view and edit the PDF files from cloud storages like Dropbox, Google Drive, OneDrive and more. You can view, edit and annotate PDF files on your iPhone and iPad. PDF Reader & Annotator by Xodo is another really good PDF which comes with notable functionalities. You can only add your signature to the documents after purchase Redact feature lets you erase or hide the sensitive information in your PDFs ![]() Supports a variety of other document formats stored locally or in the cloud Replace pictures and edit original text Edit outlines to conveniently navigate in PDF And you can also get super PDF editing features with this PDF expert after subscription. PDF Expert lets you open and read all types of documents and also helps you listen to your favourite PDF book with TTS option. PDF Expert is one of the best PDF apps on the App Store for your iPhone & iPad and it comes with lots of useful options to help you to read, highlight texts, make notes, fill forms, review documents and draw or annotate PDF documents. Scan paper documents and then convert them to the PDF format Supports viewing MS Word, Excel, PowerPoint, TXT and image files If all you need is a simple reader for daily use that doesn’t take up a lot of space on your devices, Foxit can be a great choice. With the help of cloud support, you can upload and download PDF files from popular storage providers. >Click here to learn more about filling out documents.įoxit PDF Reader is another recommendation for average users, the free version of this app comes with some basic functions like PDF viewing, annotating while the premium functions such as the TTS feature, password setting and many more are locked. Built-in OCR function helps recognize and extract text in 50+ languages Select page range first and extract images inside the PDF Create PDF from images, blank pages, clipboard content or from files browser Convert PDF to and from Word, PPT, Excel, JPG, PNG, HTML and more Sync your file to iCloud, Google Drive, Dropbox and other remote servers Support various reading options including slideshow, text reflow, auto scroll, split view, multi-tab viewer Open and view MS Office files, iWork, html, images, audios, videos and more ![]() PDF Reader Pro helps optimize your work and lets you become more productive in no time. With a few clicks, you can read and annotate your content, edit and manage PDFs, fill PDF forms, Merge PDFs, backup your files via iCloud or other storage services. It’s an extremely lightweight, fast, clean, and intuitive professional reader that allows you to open all mainstream file formats. PDF Reader Pro is a top PDF reader app which competes directly with the Adobe Acrobat Reader. Loads of features means a bigger size than most PDF readers Collaborate with others by tracking changes or using comments Easily zoom in on text or images for a closer view View and annotate the PDF files that you stored locally or various cloud services The best thing overall about the app though is the name and support behind it. The application comes with a complete tool with all of the functions you could possibly need and a simple UI. It is the global standard for reliably viewing, annotating and signing PDFs. The reason is simple because PDF format was actually developed by Adobe back in 1993. Inarguably, Adobe Acrobat Reader is the most popular PDF reader, not only on iOS platform but also on any other platforms. In this article, we’ve listed the best PDF Reader apps for your iPad and iPhone which can help you complete PDF related tasks easily and quickly. PDF is one of the most popular and widely used file formats and as more people are working and studying on the go, it is important to have a good PDF reader app installed on your mobile devices. ![]() Comes with USB charging cable and replacement tip.Fine point tip for precise writing and drawing.The pen also comes with a USB charging cable and a replacement tip for added convenience. It features a fine point tip that allows for precise writing and drawing, and comes with a built-in battery that lasts for up to 10 hours of continuous use. The Chilison Active Stylus Digital Pen is a high-quality stylus pen that is compatible with the Fire HD 10 and other tablets and smartphones. Sleek, anodized aluminum body with a clipġ.9mm fine point tip that ensures high accuracy and precision Provides the best quality and performance The pen is compatible with all touch screen devices, including the Fire HD 10. It has a sleek, anodized aluminum body with a clip that makes it easy to carry around. The pen has a 1.9mm fine point tip that ensures high accuracy and precision while writing or drawing. The Adonit Dash 3 Stylus Pen is a premium option for those who want the best quality and performance. Prosįine point fiber tip that ensures high accuracy and precision The pen is compatible with all capacitive touch screen devices, including the Fire HD 10. The pen has a sleek aluminum body with a clip that makes it easy to carry around. It has a fine point fiber tip that ensures high accuracy and precision. The Mixoo Capacitive Stylus Pen is another excellent option for Fire HD 10 tablets. The pen is relatively heavier compared to other stylus pens Retractable ballpoint pen at the other end Soft, rubberized tip that glides smoothly It also has a retractable ballpoint pen at the other end that can be used for writing on paper. The pen has a soft, rubberized tip that glides smoothly over the screen and prevents scratches. The AmazonBasics Executive Stylus Pen is a popular choice for those who want a budget-friendly option without compromising on quality. The cap is not attached to the pen, so it can be easily misplacedĬompatible with all capacitive touch screen devices The pens have a lightweight aluminum body with a clip that makes them easy to carry around. ![]() They are compatible with all capacitive touch screen devices, including the Fire HD 10. These pens have a 1.5mm fine point fiber tip that provides high precision and accuracy while writing or drawing. Whether you're trying to load a Prime Original or look for cookie recipes to start baking, Show Mode gives you everything you need to use your Fire Tablet hands-free.The MEKO Universal Stylus Pens are one of the best options for Fire HD 10 tablets. A swipe to the right opens a control panel with quick commands while your homescreen rotates between the clock, weather, news, and more. If you have an Echo Show in your house, you'll feel at home with this UI. You can access Show-specific settings by tapping the gear icon in the notification panel while this mode is enabled. Otherwise, issuing voice commands is harder. It works best when Hands-Free Mode is also turned on. To use Show Mode, you must enable Alexa on your device. Toggle on Show Mode, and right before your eyes, your Fire Tablet transforms into an Echo Show, complete with a large-screen UI. Just grab your tablet stand, prop it up on a flat surface, then swipe down from the top of the device to open the Quick Settings menu. It's the perfect option if you've got a road trip coming up.ĭid you know Fire HD 8 and Fire HD 10 devices can double as makeshift Echo Show alternatives? It transforms your tablet into the perfect gadget for the kitchen counter, the coffee table in the living room, or anywhere else around your house. There's also a filter to display downloaded content only, in case you're offline for an extended period of time. This includes synced Kindle purchases, apps and games from the Appstore, Prime Video content, and Audible selections, and you can customize it using the button at the bottom of this page. The Library tab is where you'll spot all of your digital content. It might not be immediately apparent, but you can easily create folders by dragging apps on top of each other or uninstall (most) unwanted software by pressing and holding an icon. You can get rid of advertisements or suggested content on the top of the Home tab by tapping, holding, and selecting Not Interested, leaving only your list of installed apps. With three tabs (For You, Home, and Library), Amazon's setup is simplified for a big-screen experience. Amazon's homescreen experience may not feel like classic Android, but with a little customization, it's usable, especially if you're a Prime subscriber. ![]() It's a difficult process that's easily broken through system updates and reboots. Although setting a third-party launch on Fire OS is possible, we don't recommend it. ![]() Ranaldo’s Karen Revisited opens wide into a blistering din, gnarled feedback and static blossoming into a delicate ambient murmur. And his Radical Adults Lick Godhead Style, a paean to generations of New York kids surviving only on their own creative fuel, unspools around a tense, stabbing theme, as dark and dramatic as the best of what Interpol were offering. Opener The Empty Page is one of Moore’s most ready-made earworms, its riff versatile enough for an explosive solo and the crystalline bridge that follows. Murray Street, recorded in autumn 2001 in their Manhattan studio, close to Ground Zero, might be Sonic Youth’s most staggering guitar album, with Jim O’Rourke helping them realise their angular jam-band dreams. Listen to Sister, and you hear those inchoate ideas emerging. In the late 80s, Moore talked about incorporating elements of pop into Sonic Youth, which became more obvious in the decade to come. ![]() Their prowling cover of Hot Wire My Heart is one of the more footloose moments in their catalogue, Crime’s punk anthem barbed with plangent guitars and blown-out bass. If the Pacific Coast Highway evokes images of California’s wondrous coastline, Gordon’s song of the same name makes you fear for the hitchhiking kid on the side of the road, thumb out as the predator pulls up. Sister’s 10 songs continually fuse melee with melody, danger with delight. What’s more, Steve Shelley, a drummer with a preternatural sense for the way the messes Sonic Youth made would move, had settled into his role. In 1987, they were stepping away slowly from their early no-wave pastiche and their purely bracing tirades, and moving toward the art-rock intricacy and coded accessibility that would remain their hallmarks. Urgent but articulate, Sister captures Sonic Youth at a quintessential crossroads. Sonic Youth: Beauty Lies in the Eye – video If you get from the perfect Teen Age Riot to the powerful Kissability without hearing Sonic Youth’s fundamental songwriting prowess, start again. And Lee Ranaldo, the band’s classic rock doyen, pulls Jimi Hendrix and Joni Mitchell into Hey Joni, a song balanced by bright harmonics and shrieking distortion and driven by a sense of trying to outrace the past. One track later, Kim Gordon’s The Sprawl, inspired by the writing of William Gibson, lulls you with chanted vocals before the guitars split into bedlam, scoring a dystopian daydream. During its first seven songs alone, Thurston Moore lets Silver Rocket, an explosive rock hit waiting in the wings, collapse in a conflagration of smoking amps. Essentially from the start, though, three distinctive songwriters powered the band in tandem, creating records that felt like rollercoasters – perhaps none more so than Daydream Nation. Sonic Youth sometimes get a reductive rap for just making a racket. This is the record that would rightfully earn Sonic Youth a major-label deal and a spot in the United States’ National Recording Registry. ![]() ![]() During 70 breathless minutes, Sonic Youth drift through refracted spoken-word and musique concrète collage, churn through Swans-like rumblings and atonal barrages, and shimmer through a series of pop-rock gems lined with shards of dissonance and sheets of feedback. One of indie rock’s truly sacred texts, Daydream Nation is both a gripping synthesis of the punk, college rock and modern composition that surrounded the young New York band, and a living mood board for so much of what would arrive in the decades to come. It’s fiddly rather than infuriating, and you do get used to it. TALK WE ARE ALL HACKERS (2016) Born and raised in Northern Ireland. It’s understandable, though, given that we’re trying to replicate the classic mouse-and-keyboard FPS setup on a handheld touchscreen. Super Hexagon is a minimal action game by Terry Cavanagh, with music by Chipzel.Super Hexagon was a best of 2012 runner up for game of the year on the app store.'A masterpiece of design purity' - EDGE 9/10'This is the kind of game you’re going to pass off to your friends and say, You’ve gotta check this out. FIRST INDUSTRY PROJECT: SUPER HEXAGON (2012) FAVORITE INDUSTRY PROJECT. We got our hands in a bit of a muddle while getting the hang of it all. You get the option of having two fire buttons, and we’d recommend this, since we found it necessary to switch between left and right thumb shooting depending on the circumstances: depending on whether you’re strafing or tracking a moving target, you’ll have a different finger free to shoot with, and if you use the ‘tap to lock on’ function, that adds additional complication. These Super Hexagon cheats are designed to enhance your experience with the game. The shooting took us a while to get used to on a touchscreen, for one thing. Our Super Hexagon +1 trainer is now available and supports STEAM. Deus Ex: The Fall review: CombatĬombat in The Fall isn’t perfect, cathartic as those close-up animations can be. And there’s something really satisfying about exploring the levels and discovering semi-hidden routes – or hacking your way through security gates, as we’ll discuss later – that get you the drop on the villains. Here are some of the NEW features to be excited about Brand new level editor (documentation. ![]() Rewritten with plenty of new features, Super Haxagon will provide you with hours of entertainment on your homebrewed or cfw'd 3DS. It runs full speed on the old 3DS, new 3DS, and in citra-emu/citra. Start the game with controller unplugged, from joypad settings, select Customize controls, then Disable joypad.Start the game with controller inserted, Enable joypad and immediately bind the buttons properly. It’s genuinely open to multiple approaches. Super Haxagon is a Super Hexagon clone for the 3DS. Because of default bindings, results unusable menus. Or you can go in guns blazing like Arnie in Commando. ![]() So you can sneak around, picking off peripheral lookouts before going in for the kill. ![]() Here's the list features.The enemies nearby appear on your map as vector arrows, colour-coded to indicate how aware they are of your presence. (Hopefully it's fine to have two threads about this) A variety of mods are available, which extend the gameplay by a large margin Pixel Dungeon is hard Tons of replayability thanks to the randomly generated. 2073SuperflightRetro City RampageCAGEDuck GameReturn to Castle WolfensteinProject HighrisePapers, PleaseGeometry DashHacknetSonic ManiaSuper Hexagon. There's also a thread about it for the 3DS users if you want to run it there. This is the discussion thread for running Super Haxagon on your Nintendo Switch. The result is an open-source version of the game that works on the Nintendo Switch, 3DS, and PC. ![]() Anyway, around a month ago I decided to re-write the entire thing in C++ so it's cross platform. Cheatbook is the resource for the latest Cheats, tips, cheat codes, unlockables, hints and secrets to get the edge to win. It's was a Super Hexagon recreation for the 3DS featuring custom levels and entertaining game play. Important facts about Hexagons: Hexagons are third order permutohedrons. Around 4 years ago I developed a 3DS homebrew called "Super Haxagon". Super Hexagon is a minimal action game by Terry Cavanagh, with music by Chipzel. ![]() With our current understanding of microbiology and infection, it would be difficult to comprehend why faeces and excrement, rich in disease causing pathogens, was used in the open wounds caused by burn. A more recent example can be found in the 19 th century, where a mixture of linseed oil and lime-water (termed Carron oil) was being used to treat burns in Ironworkers in Scotland. The first description of first aid for burns was around 854 CE-925 CE, by Muhammad ibn Zakariya al-Razi (or otherwise known as Rhases in the west), an Arabian physician who recommended cold water for the relief of pain from burns. Many famous philosophers and physicians have contributed to burn wound management such as Hippocrates, who in 400 BC, described the use of bulky dressings impregnated with rendered pig fat and resin with alternated warm vinegar soaks, augmented with tanning solutions made from oak bark and in the 1 st century AD, Celsus described the use of wine and myrrh as a burn lotion, which had bacteriostatic properties. In 600 BC, Chinese described the use of tea leave abstracts and tinctures for burns. One of the earliest records of burn treatment was described in an Egyptian Smith Papyrus written in 1600 BC which advocated the use of resin and honey salve for treating burns, and the Ebers Papyrus in 1500 BC described the use of a wide variety of substances to treat burn wounds. The majority of ancient burn care consisted of topical therapies and can be traced back to centuries. It was not until the past 50 years that the mortality of burns has been dramatically improved, thanks to the better understanding of the patho-physiology of burn injury. However, this was not reflected in improving survival and many patients still died of shock and infection. There was an exponential increase in biomedical research and knowledge from the 18 th to early 20 th century in burn care, such as the recognition of the importance of burn surface area and skin grafting by Reverdin. ![]() Physicians have searched for and formulated a myriad of treatments for burns over the centuries but these treatments mostly were of little benefit to the victims mainly because the fundamental understanding of the patho-physiological impact of burns was not known yet. Burns are still one of the top causes of death and disability in the world. We also briefly discuss some future directions in burn care such as the use of cell and pharmalogical therapies.īurn injuries are amongst one of the most devastating of all injuries, having a great impact on the patients physically, physiologically and psychologically. The introduction of metabolic care, nutritional support and care of inhalational injuries further improved the outcome of burn patients. Advancements in the methods of assessing the surface area of burns paved way for more accurate fluid resuscitation, minimising the effects of shock and avoiding fluid over-loading. This along with the advent of antiseptic surgical techniques, burn depth classification and skin grafting allowed the excision and coverage of full-thickness burns which resulted in greatly improved survival rates. ![]() The use of systemic antibiotics and topical silver therapy greatly reduced sepsis related mortality. Most major advances in burn care occurred in the last 50 years, spurred on by wars and great fires. In this article we look back at how the treatment of burns has evolved over the centuries from a primarily topical therapy consisting of weird and wonderful topical concoctions in ancient times to one that spans multiple scientific fields of topical therapy, antibiotics, fluid resuscitation, skin excision and grafting, respiratory and metabolic care and nutrition. Burn injuries are one of the most common and devastating afflictions on the human body. ![]() This study attempts to estimate annual vehicle kilometers traveled (AVKT) per privately-owned passenger vehicle and their daily distance patterns by vehicle class and geographic region. ![]() Given the explosive growth of the passenger vehicle market and energy demands in China, research on vehicle-use intensity and driver-travel patterns is critical for better assessing travel demand and its implications for alternative fuel vehicles, energy security, and environmental policies. The following NTHV information is presented: the results of the trade-off studies are summarized the overall vehicle design the selection of the design concept and the base vehicle (the Chevrolet Citation), the battery pack configuration, structural modifications, occupant protection, vehicle dynamics, and aerodynamics the powertrain design, including the transmission, coupling devices, engine, more » motor, accessory drive, and powertrain integration the motor controller the battery type, duty cycle, charger, and thermal requirements the control system (electronics) the identification of requirements, software algorithm requirements, processor selection and system design, sensor and actuator characteristics, displays, diagnostics, and other topics environmental system including heating, air conditioning, and compressor drive the specifications, weight breakdown, and energy consumption measures advanced technology components, and the data sources and assumptions used. The NTHV uses a modified GM Citation body, a VW Rabbit turbocharged diesel engine, a 24KW compound dc electric motor, a modified GM automatic transmission, and an on-board computer for transmission control. The derivation of and actual preliminary design of the Near Term Hybrid Vehicle (NTHV) are presented. more » From this study it is concluded that hybrid vehicles are best suited for short range driving the life cycle cost of the preliminary design near term HV is 25% higher than that for a ICE vehicle the availability of essential materials such as battery leads needs investigation and further evaluation of the effects of acute petroleum shortages as well as increased fuel prices and of the desirability of various sizes of HV's is required. The reliability and safety, life cycle costs, and computerized simulation of vehicle performance are discussed. Using a modified General Motors X-body car (Citation) as the base vehicle, the preliminary design of the vehicle, the power train, including internal combustion engine, batteries, and transmission, and the electronic control system are described. After analyzing automobile usage in terms of trip lengths and purposes, the petroleum savings that could result from using hybrid versus conventional passenger vehicles were estimated. = ,Īctivities in Phase I of a program to develop a preliminary design of a hybrid (electric and internal combustion engine powered) vehicle are summarized. ![]() ![]() Multiple gels may be electrotransferred in the standard field option, which is performed either at constant current (0.1 to 1 A) or voltage (5 to 30 V) from as little as 1 hour to overnight. The supported gel sandwich is placed vertically in a tank between stainless steel/platinum wire electrodes and the tank is filled with transfer buffer. The gel is then placed in the “transfer sandwich” (filter paper-gel-membrane-filter paper), cushioned by pads and pressed together by a support grid. When performing a wet transfer, the gel is first equilibrated in transfer buffer. The efficiency of protein transfer can be affected by the chemistry, thickness of the gel, the molecular weight of the proteins being transferred, the type of membrane and transfer buffers used, and the transfer method. In addition to the challenges of immunodetection in the protein blotting workflow, the transfer of proteins from a gel matrix to a membrane is a potential hurdle. An appropriate method is then used to detect the localized probe to document the location and relative abundance of the target protein. The transferred protein is then probed sequentially with antibodies and detection probe (e.g., enzyme, fluorophore, isotope). Better target protein accessibility on the membrane by macromolecules like antibodiesĪfter transfer, the membrane must be blocked to prevent non-specific binding of the antibody to the membrane surface.Better handling capability offered by the membrane compared to a fragile gel. ![]() Protein transfer from gel to membrane is necessary for two reasons: In procedures where protein separation is not required, the sample may be directly applied to the membrane by spotting using an approach called dot blotting. After electrophoresis, the separated proteins are transferred, or "blotted", onto a solid support matrix, usually a nitrocellulose or polyvinylidene difluoride (PVDF) membrane. The first step in a western blotting procedure is to separate the proteins in a sample by size using denaturing gel electrophoresis (i.e., sodium dodecyl sulfate polyacrylamide gel electrophoresis or SDS-PAGE) or native PAGE. On the citrus front, Parker & Quinn’s blood orange margarita uses the “unique citrus from Italy” to flavor its take on the cocktail, which also gets an added boost of spice thanks to some ginger beer. For example, Meg McCabe, the assistant general manager of Park Avenue Tavern in New York City, tells Us Weekly the restaurant’s passion fruit margarita uses the exotic fruit to give their version of the drink a “vibrant color” and flavor profile that will let your taste buds “come alive.” Though all you need to make a marg is tequila, orange liqueur and lime juice, there are countless ways to add additional flavor to the popular libation. In other words, it’s about time to kick your feet back and honor the drink by whipping up one of your own. National Margarita Day is upon us! The annual holiday, which falls on Friday, February 22, celebrates the beloved drink which, according to Nielsen data, is officially America’s favorite cocktail. Need more inspiration for what to throw together in celebration of National Margarita Day? Scroll down for some tasty and simple recipes!Ĭredit: Jenna Murray In Good Company Hospitality(2)Ĭelebrate National Margarita Day With These Tasty Drink Recipes Sign up for Us Weekly's free, daily newsletter and never miss breaking news or exclusive stories about your favorite celebrities, TV shows and more! As Masso sees it, the tart flavor and effervescent nature of the champagne complements the “sweet, honey-like” notes present in tequila. This drink, which was developed with some help from the Brooklyn Museum, pays tribute to Mexican artist Frida Kahlo and her “love” of the tropical fruit.įor something a bit different, Dre Masso, who is one of the original creators of the Olmeca Altos tequila brand, suggests topping your margarita off with a splash of champagne. If you’re in the market for a slightly high-brow version of a margarita to mark the holiday with, look no further than the weeping coconut margarita created by Cuervo Tradicional – the makers of the original agave-based tequila. Garnish each margarita with a lime wheel, a few pomegranate seeds and serve.In order to view the video, please allow Manage Cookies Working in batches, shake the mixture in a large ice-filled shaker, then strain into the glasses. In a pitcher, stir the remaining 1 ½ cups of pomegranate juice, the tequila, triple sec and lime juice. The glass rim into the salt until coated. Moisten the rim of 8 margarita glasses with the pomegranate juice, then dip Pour 1/4 cup of the pomegranate juice into a saucer spread kosher salt in another saucer. Ingredients Shaken & strained over ice, topped with Goslings ginger beer. Blackstone's "Irish margarita"ġ2oz glass, rimmed with fresh lime & green sugar. Add the remaining ingredients and shake vigorously for 15 seconds and strain. To mix drink: Place the cilantro in the cocktail shaker and muddle. The sweetness of the oranges pairs well with the smoky, grilled flavors of the meat, while the sourness of the drink cuts through. The orange flavors in the blood orange margarita are the perfect complement to savory dishes like grilled chicken or steak fajitas. Glass rimmed with Tajin citrus chile salt This margarita is perfect for enjoying with your favorite Uncle Julio’s dishes. ![]() Shake vigorously for 15 seconds and strain over fresh ice. To mix drink: Place all ingredients in a cocktail shaker. Let rest for 5 days to fully infuse the flavor. Let rest for 5 days to fully infuse the flavor.įor Cucumber Infused Tequila: Place half of a medium sliced cucumber in a 750ml bottle of your favorite Silver Tequila. Shake vigorously for 15 seconds and strain over fresh ice.įor Jalapeno Infused Tequila: Place one jalapeno, cut into slices, in a 750ml bottle of your favorite Silver Tequila. Garnish with a fresh or dried orange slice, if desired. Shake until very cold and strain into the prepared glass. Delicious dishes include blood orange and Mexican avocado tostada paired with a ros. Add the juices, simple syrup, and tequila to a cocktail shaker with ice. To mix drink: Place all ingredients in a cocktail shaker. Ella Canta has launched a new margarita menu ahead of the tequila. |
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